Kangangamashumi eminyaka, i-PDRN ibixhomekeke ekutsalweni kwiiseli zokuzala zesalmon. Le ndlela yendabuko ithintelwe kukuguquguquka kokunikezelwa kweentlanzi, ulandelelwano lwe-DNA olungaqhelekanga, kunye nemingeni ekulawuleni ubumsulwa—okwenza kube nzima ukuqinisekisa ukuhambelana kwexesha elide, ukwandiswa, kunye nokuthotyelwa kwemithetho.
ZethuI-Recombinant PDRNyaphuhliswa ukuze yoyise le mida yesakhiwo ngokusebenzisa ubunjineli obuphambili be-bio.
Ayinazo izilwanyana, yakhelwe kwi-biosynthesis elawulwayo
Ukusebenzisa i-E. coli DH5α njengeqonga lemveliso yebhayoloji, ulandelelwano oluthile lwe-PDRN luziswa ngee-recombinant vectors kwaye luphindaphindwe ngokufanelekileyo ngokubiliswa kweentsholongwane.
Le ndlela isusa ukuxhomekeka kwizinto ezifumaneka kwiintlanzi, ijongana nokungazinzi kobonelelo kunye neenkxalabo zokhuseleko lwemvelaphi yezilwanyana kwindawo ezifumaneka kuyo, ngelixa ihambelana nemigangatho engqongqo yolawulo kwi-EU, e-US, nakwiimarike zehlabathi.
Kwangaxeshanye, imveliso ihlalaIsekelwe kwi-DNA kwaye yenziwe ngendalo, okwenza kubeindlela ye-vegan, engeyoyezilwanyana, kodwa eyinyani ngokwebhayolojiukuya kwi-PDRN yendabuko ephuma kwi-salmon.
Ulandelelwano olucwangciswe ngokuchanekileyo, hayi ukukhupha ngokungacwangciswanga
Ngokungafaniyo ne-PDRN eqhelekileyo efunyenwe ngokukhupha okungakhethiyo, iteknoloji ye-recombinant ivumelaulawulo olupheleleyo kulandelelwano lwe-DNA kunye nobude beziqwenga.
Ulandelelwano olufutshane lungayilwa kwizicelo zokulwa nokuvuvukala
Ulandelelwano lwemixokelelwane ephakathi ukuya kwende lunokulungiswa ukuze luxhase ukuvuselelwa kwe-collagen kunye nokulungiswa kolusu
Olu tshintsho—ukusuka ekukhutshweni okungacwangciswanga ukuya kwi-biosynthesis ekujoliswe kuyo—luvula amathuba amatsha ophuhliso oluqhutywa ngumsebenzi kunye neefomyula ezenziwe ngokwezifiso.
Ukukhula kunye nokuphindaphinda kwinqanaba lemizi-mveliso
Ngokudibanisa utshintsho oluphuculiweyo lobushushu kunye nokulungiswa kweeseli okufanelekileyo, ukufunxwa kweplasmid kunye nemveliso kuphucuka kakhulu.
Idityaniswe nokucheba umzimba okunezinyathelo ezininzi kunye nokucocwa kwe-chromatographic okucwangcisiweyo, le nkqubo iyaqhubeka nokufezekisaubumsulwa be-biomedical-grade (≥99.5%).
Iiparamitha zokubilisa ezisemgangathweni ziqinisekisa ukwanda okuthe tye ukusuka kwimveliso yovavanyo ukuya kwimveliso yorhwebo.
Ukusebenza kakuhle kuqinisekiswe yidatha yangaphambi kweklinikhi
Izifundo zangaphambi kweklinikhi zibonisa ukuba i-PDRN ephinda i-recombinant inikaukuvuselelwa okuphezulu kokwenziwa kwe-collagen yohlobo lwe-1 lomntuxa kuthelekiswa nee-PDRN kunye nee-DNA-metal complexes eziqhelekileyo ezivela kwi-salmon.
Ezi ziphumo zixhasa ukusetyenziswa kwayo ekulungiseni ulusu nasekulweni nokwaluphala, zibonelelaisisombululo sezithako esilandelekayo sedatha, esiqhutywa yindlela yokusebenza.
I-Recombinant PDRN ayisiyonto ithatha indawo yayo—luphuculo lwetekhnoloji.
Ngokudibanisa uyilo oluchanekileyo lwe-sequence kunye ne-biosynthesis elawulwayo, iteknoloji ye-recombinant iphucula i-PDRN bioactivity ngelixa ibonelelaenye indlela ezinzileyo, ye-vegan, neyendaloukuya kwi-PDRN ephuma kwizilwanyana—ukubeka umlinganiselo omtsha wezithako zokuvuselela ulusu zesizukulwana esilandelayo.
Ixesha leposi: Disemba-31-2025